Structural & Molecular Biology

Blotting Techniques

Blotting is the fashion in which nucleic acids or proteins are paralyzed onto a solid support generally nylon or nitrocellulose membranes. Blotting of nucleic acid is the central fashion for hybridization studies. Nucleic acid labeling and hybridization on membranes have formed the base for a range of experimental ways involving understanding of gene expression, association etc. Blotting ways are among the most common approaches used in a molecular biology laboratory. These ways, Southern, northern, and immune blotting, are applicable to a variety of macromolecules including DNA, RNA, and protein, independently. Each of the ways are dependent on the capability to resolve the individual macromolecules in a size  dependent manner, transfer the motes to a solid support, and eventually use a defined inquiry to descry the specific patch of interest.